Bilirubin oxidation in dmso and chloroform
| 18.08.2018, 07:55 | |
In 1998 it was found that bilirubin may serve as an antioxidant in the tissues of the heart and nerves, though the manifestation mechanism of this function is still unknown. Moreover, understanding the regularities of free radical reactions under the hyperbilirubinemia is the key to the development of criteria for differential diagnosis followed by jaundice. The aim of this research was to study the bilirubin oxidation in different model systems. Building kinetic models of the process will help to establish the mechanism of antioxidant action of the tetrapyrrolic pigment. Kinetic parameters of the oxidation reaction depending on the solvent nature and the oxidant concentration were also compared. Chloroform and dimethylsulfoxide were used as model media simulating the non-aqueous lipid layers of cells. Benzoyl peroxide was used to generate long-lived free radicals. The most probable mechanism of tetrapyrrolic pigment oxidation seemed to be connected with the possibility of organic radicals’ interactions with methylene spacer of bilirubin. It should be noted that the mechanisms of the oxidation reactions in DMSO and chloroform are fundamentally different from each other. Acknowledgement: This study was supported by the Russian Foundation for Basic Research (Project No. 12-03-31309). Serebryakova M. K. | |
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